Journal: Advanced Exercise and Health Science

Article Title: Exercise-Triggered Cardiac Remodeling: AKIP1 as a Novel Mediator of Physiological Hypertrophy

doi: 10.1016/j.aehs.2025.05.002

Figure Lengend Snippet: Fig. 1. Changes in cardiac function after exercise in mice. (A) Ultrasound and electrocardiogram (ECG) images of mice. (B) Indices of cardiac function in mice. Con group: control group; EX group: exercise group; shAAV-AKIP1 +EX group: interference with AKIP1 adeno-associated virus expression in vivo injection+exercise group. LVMI: left ventricular mass index; LVID-d: left ventricular end-diastolic internal diameter; LVID-s: left ventricular end-systolic internal diameter; LVPW-d: left ventricular posterior wall thickness-diastolic; LVPW-s: left ventricular posterior wall thickness-systolic; EF: ejection fraction; FS: left ventricular shortening fraction. * indicates p < 0.05 compared with con group; ** indicates p < 0.01 compared with con group, ## indicates p < 0.01 compared with EX group.

Article Snippet: The protocol for immunoblot analysis has been previously described.23 And the blot was incubated with primary antibodies such as GAPDH (Cat # 5174, Cell Signaling Technology Inc., MA, USA), AKIP1 (Cat # 5203, Cell Signaling Technology Inc., MA, USA).

Techniques: Control, Virus, Expressing, In Vivo, Injection

Journal: Advanced Exercise and Health Science

Article Title: Exercise-Triggered Cardiac Remodeling: AKIP1 as a Novel Mediator of Physiological Hypertrophy

doi: 10.1016/j.aehs.2025.05.002

Figure Lengend Snippet: Fig. 2. Evaluation of animal models of exercise-in duced cardiac hypertrophy. (A) Representative photo micrographs of mice myocardial tissue stained with hematoxylin and eosin (HE). (B) ratio of heart weight to tibial length (HW/TL) in mice. (C) Expression of mRNA for markers of cardiac hypertrophy in mice. Con group: control group; EX group: exercise group; shAAV-AKIP1 +EX group: interference with AKIP1 adeno-associated virus expression in vivo injection +exercise group. ANP: natriuretic peptide A; BNP: brain natriuretic peptide; α-actin: α-smooth muscle actin; SERCA-2α: sarcoplasmic reticulum/endoplasmic reticulum calcium ATPase 2α; α-MHC: α-myosin heavy chain; β-MHC: β-myosin heavy chain. * indicates p < 0.05 compared with con group; ** indicates p < 0.01 compared with con group, ## indicates p < 0.01 compared with EX group.

Article Snippet: The protocol for immunoblot analysis has been previously described.23 And the blot was incubated with primary antibodies such as GAPDH (Cat # 5174, Cell Signaling Technology Inc., MA, USA), AKIP1 (Cat # 5203, Cell Signaling Technology Inc., MA, USA).

Techniques: Staining, Expressing, Control, Virus, In Vivo, Injection

Journal: Advanced Exercise and Health Science

Article Title: Exercise-Triggered Cardiac Remodeling: AKIP1 as a Novel Mediator of Physiological Hypertrophy

doi: 10.1016/j.aehs.2025.05.002

Figure Lengend Snippet: Fig. 3. Expression of related gene in the model of exercise-induced cardiac hypertrophy. (A) The level of cardiac AKIP1 mRNA in mice. (B) The level of cardiac AKIP1 protein in mice. (C) The level of cardiac PI3K(p110α) mRNA in mice. (D) The level of cardiac mTORC1 mRNA in mice. Con group: control group; EX group: exercise group; shAAV-AKIP1 +EX group: interference with AKIP1 adeno-associated virus expression in vivo injection+exercise group. AKIP1: a kinase interacting protein 1; PI3K(p110α): the p110α isoform of phosphatidylinositol-3-kinase; mTORC1: mechanistic target of rapamycin complex 1. ** indicates p < 0.01 compared with con group, ## indicates p < 0.01 compared with EX group.

Article Snippet: The protocol for immunoblot analysis has been previously described.23 And the blot was incubated with primary antibodies such as GAPDH (Cat # 5174, Cell Signaling Technology Inc., MA, USA), AKIP1 (Cat # 5203, Cell Signaling Technology Inc., MA, USA).

Techniques: Expressing, Control, Virus, In Vivo, Injection